Madrid, Madrid, Spain, 5 May 2016 | AAP

Nick Kyrgios has knocked world No.4 Stan Wawrinka out of the Mutua Madrid Open in the second round.

Kyrgios took an hour and 48 minutes to down Wawrinka 7-6(7) 7-6(2) on Wednesday, at one stage raging at the chair umpire over a line call.

The official stuck with his decision in the first set tie-breaker after leaving his chair to examine the clay for a ball mark, but Kyrgios maintained focus.

The Aussie closed out the first set and continued to produced a display of both power-laden yet efficient tennis to score his second win over the two-time major champion.

The 21-year-old Kyrgios next faces Pablo Cuevas, who overcame 13th seed Gael Monfils in three sets.

Aussie women advance

Earlier on Wednesday, Daria Gavrilova claimed a massive scalp to win through to the quarterfinals of the claycourt tournament in the Spanish captial.

The young Aussie scored a straight-sets victory over two-time Wimbledon winner Petra Kvitova, the defending champion in Madrid who was seeded fifth.

It was Gavrilova’s second straight victory over the Czech after beating her in straight sets at Australian Open 2016.

The Australian accounted for Britain’s Heather Watson 7-5 6-4 in the opening round in Madrid before impressing again with a 6-2 7-6(4) win over No.12 Elina Svitolina of Ukraine.

The draw has opened up; with in-form No.4 seed Victoria Azarenka withdrawing from the tournament, Gavrilova instead faces American qualifier Louisa Chirico for a place in the semifinals.

Meanwhile, Sam Stosur triumphed in a gruelling third-round clash over No.8 seed Carla Suarez Navarro.

Stosur fought back from a set down to beat the Spanish world No.11 4-6 6-2 6-3 in the match that lasted just under two hours.

She next plays the winner of a match between Romanian Patricia Tig and Madison Keys from the US.